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Synapse. 2018 Sep;72(9):e22041. doi: 10.1002/syn.22041. Epub 2018 Jul 20.

Test-retest variability of [11 C]ABP688 estimates of metabotropic glutamate receptor subtype 5 availability in humans.

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Department of Psychiatry, McGill University, 1033 Pine Ave W, Montreal, Quebec, H3A 1A1, Canada.
Department of Neurology and Neurosurgery, McGill University, Montreal Neurological Institute, 3801 University Ave, Montreal, Quebec, H3A 2B4, Canada.
Translational Neuroimaging Laboratory, McGill University Research Centre for Studies in Aging, 6825 Boulevard LaSalle, Verdun, Quebec, H4H 1R3, Canada.


[11 C]ABP688 is a positron emission tomography (PET) radioligand that binds selectively to metabotropic glutamate type 5 receptors (mGluR5). The use of this tracer has identified receptor binding changes in clinical populations, and has been informative in drug occupancy studies. However, previous studies have found significant increases in [11 C]ABP688 binding in the later scan of same-day comparisons, and estimates of test-retest reliability under consistent scanning conditions are not available. The objective of this study was to assess the variability of [11 C]ABP688 binding in healthy people in scans performed at the same time of day. Two [11 C]ABP688 scans were acquired in eight healthy volunteers (6 women, 2 men) using a high-resolution research tomograph (HRRT). Scans were acquired 3 weeks apart with start times between 10:00am and 1:30pm. Mean mGluR5 binding potential (BPND ) values were calculated across cortical, striatal and limbic brain regions. Participants reported on subjective mood state after each scan and blood samples were drawn for cortisol analysis. No significant change in BPND between scans was observed. Variability in BPND values of 11-21% was observed across regions, with the greatest change in the hippocampus and amygdala. Reliability was low to moderate. BPND was not statistically related to scan start time, subjective anxiety, serum cortisol levels, or menstrual phase in women. Overall, [11 C]ABP688 BPND estimates show moderate variability in healthy people. Reliability is fair in cortical and striatal regions, and lower in limbic regions. Future research using this ligand should account for this in study design and analysis.


[11C]ABP688; metabotropic glutamate receptor type 5; positron emission tomography; test-retest


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