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Int J Mol Sci. 2018 Jun 21;19(7). pii: E1828. doi: 10.3390/ijms19071828.

The Antioxidant and Anti-Inflammatory Activities of 8-Hydroxydaidzein (8-HD) in Activated Macrophage-Like RAW264.7 Cells.

Author information

1
Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Korea. im144069@gmail.com.
2
Basic Research & Innovation Division, R&D Center, Amorepacific Corporation, Yongin 17074, Korea. kangyg82@amorepacific.com.
3
Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Korea. kjhmlkhjml@hanmail.net.
4
Basic Research & Innovation Division, R&D Center, Amorepacific Corporation, Yongin 17074, Korea. jaykim@amorepacific.com.
5
Basic Research & Innovation Division, R&D Center, Amorepacific Corporation, Yongin 17074, Korea. trlee@amorepacific.com.
6
Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Korea. bioneer@skku.edu.
7
Basic Research & Innovation Division, R&D Center, Amorepacific Corporation, Yongin 17074, Korea. dhkim417@amorepacific.com.
8
Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Korea. jaecho@skku.edu.

Abstract

8-Hydroxydaidzein (8-HD) is a daidzein metabolite isolated from soybeans. This compound has been studied for its anti-proliferation, depigmentation, and antioxidant activities. However, the anti-inflammatory activities of 8-HD are not well-understood. Through its antioxidant effects in ABTS and DPPH assays, 8-HD reduces the production of sodium nitroprusside (SNP)-induced radical oxygen species (ROS). By triggering various Toll-like receptors (TLRs), 8-HD suppresses the inflammatory mediator nitric oxide (NO) without cytotoxicity. We examined the regulatory mechanism of 8-HD in lipopolysaccharide (LPS)-induced conditions. We found that 8-HD diminishes inflammatory gene expression (e.g., inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and tumor necrosis factor (TNF)-α) by regulating the transcriptional activities of nuclear factor (NF)-κB and activator protein 1 (AP-1). To find the potential targets of 8-HD, signaling pathways were investigated by immunoblotting analyses. These analyses revealed that 8-HD inhibits the activation of TAK1 and that phosphorylated levels of downstream molecules decrease in sequence. Together, our results demonstrate the antioxidant and anti-inflammatory actions of 8-HD and suggest its potential use in cosmetics or anti-inflammatory drugs.

KEYWORDS:

8-hydroxydaidzein; anti-inflammation; antioxidant; soybeans

PMID:
29933606
PMCID:
PMC6073157
DOI:
10.3390/ijms19071828
[Indexed for MEDLINE]
Free PMC Article

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