Structural analysis of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina

J Biochem. 1985 May;97(5):1501-8. doi: 10.1093/oxfordjournals.jbchem.a135205.

Abstract

In the previous paper, we described the identification of two abundant mRNAs of Sarcophaga peregrina (flesh-fly) which are selectively expressed in the fat body of middle third instar larvae. One of these mRNAs was found to encode a protein with a molecular mass of about 25,000 (25-kDa protein) when translated in vitro (Tamura, H., et al. (1983) Dev. Biol. 99, 145-151). Present paper reports the nucleotide sequence of a 2.3 kb DNA containing the entire gene for the 25-kDa protein. This gene consisted of four exons and contained an open reading frame for 184 amino acids. A CAT box and a TATA box were found in the 5'-flanking sequence. A poly A addition signal of AATAAA was assigned to the non-coding region in the fourth exon. A sequence having 75% homology with SV40 enhancer core sequence was identified in the non-coding region of the first exon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue
  • Animals
  • Base Sequence
  • DNA Restriction Enzymes
  • Diptera / genetics*
  • Diptera / growth & development
  • Gene Expression Regulation
  • Genes
  • Molecular Weight
  • Proteins / genetics*

Substances

  • Proteins
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X02570