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Mar Biotechnol (NY). 2018 Oct;20(5):666-675. doi: 10.1007/s10126-018-9837-1. Epub 2018 Jun 22.

Mapping Genetic Loci for Quantitative Traits of Golden Shell Color, Mineral Element Contents, and Growth-Related Traits in Pacific Oyster (Crassostrea gigas).

Song J1,2, Li Q3,4, Yu Y1, Wan S1, Han L1, Du S5.

Author information

1
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
2
Qingdao Agricultural University, Qingdao, China.
3
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China. qili66@ouc.edu.cn.
4
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. qili66@ouc.edu.cn.
5
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.

Abstract

Golden shell color and mineral content are important economic traits of Pacific oyster (Crassostrea gigas). In this study, we mapped a series of quantitative trait loci (QTLs) that control zinc (Zn) and magnesium (Mg) content, shell color and growth performance to two sex-averaged linkage maps from the FAM-A and FAM-B families. In total, ten QTLs were identified in seven linkage groups (LGs) in the FAM-B family, and seven QTLs were identified in four linkage groups in the FAM-A family. Two QTLs affecting the trait of golden shell color were identified in LG8 of the FAM-A and LG10 of the FAM-B families, which could explain 20.2 and 10.5% of the phenotypic variations, respectively. Two QTLs for Zn content were identified that could contribute to 17.9 and 34.44% of the phenotypic variations in FAM-A. Six QTLs for Zn and Mg contents were identified in four LGs (LG1, LG2, LG5, and LG9) in FAM-B, which explained 13.5-26.7% of the phenotypic variations. In addition, seven QTLs related to oyster growth were recognized in both FAM-A and FAM-B families accounting for 14.6-36.7% of the phenotypic variations. All of the DNA markers in QTL regions were blasted and 14 genes associated with above traits were identified. The mRNA expression of these genes was determined by quantitative RT-PCR. These QTLs and candidate genes could be used as potential targets for marker-assisted selection in C. gigas breeding.

KEYWORDS:

Crassostrea gigas; Marker-assisted selection; Quantitative trait loci; Shell color; Zinc and magnesium element

PMID:
29931607
DOI:
10.1007/s10126-018-9837-1

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