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Sci Rep. 2018 Jun 21;8(1):9436. doi: 10.1038/s41598-018-27819-x.

Methodology for Y Chromosome Capture: A complete genome sequence of  Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads.

Author information

1
GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Av. Ilustracion, 114 - PTS - 18016, Granada, Spain. mjesusac@ugr.es.
2
Centro di Antropologia Molecolare per lo Studio del DNA Antico, Dipartimento di Biologia, Università degli Studi di Roma"Tor Vergata", via della Ricerca Scientifica n. 1, 00173, Rome, Italy. mjesusac@ugr.es.
3
University of Granada. Dept. of Biochemistry & Molecular Biology III - Faculty of Medicine - PTS, 18016, Granada, Spain. mjesusac@ugr.es.
4
GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Av. Ilustracion, 114 - PTS - 18016, Granada, Spain.
5
Centro di Antropologia Molecolare per lo Studio del DNA Antico, Dipartimento di Biologia, Università degli Studi di Roma"Tor Vergata", via della Ricerca Scientifica n. 1, 00173, Rome, Italy.
6
University of Granada. Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine -PTS, 18016, Granada, Spain.

Abstract

This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.

PMID:
29930304
PMCID:
PMC6013464
DOI:
10.1038/s41598-018-27819-x
[Indexed for MEDLINE]
Free PMC Article

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