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Curr Protoc Mouse Biol. 2018 Jun;8(2):e42. doi: 10.1002/cpmo.42. Epub 2018 May 21.

Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice.

Author information

1
Mammalian Genetics Unit, Medical Research Council, Harwell Science and Innovation Campus, Oxford, United Kingdom.
2
Mary Lyon Centre, Medical Research Council Harwell Science and Innovation Campus, Oxford, United Kingdom.

Abstract

Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging. To develop novel models of age-related disease, we undertook a phenotype-driven screen of a pipeline of mutagenized mice. Here, we describe some of the underlying protocols and outline important aspects to consider when studying aged mice.

KEYWORDS:

age-related disease; ageing; mutagenesis; phenotyping

PMID:
29927552
DOI:
10.1002/cpmo.42
[Indexed for MEDLINE]

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