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Cornea. 2018 Oct;37(10):1311-1317. doi: 10.1097/ICO.0000000000001671.

Therapeutic Effects of Topical 8-Oxo-2'-deoxyguanosine on Ethanol-Induced Ocular Chemical Injury Models.

Author information

1
Department of Physiology, College of Medicine, Gachon University, Incheon, Korea.
2
Gachon University College of Medicine, Incheon, Korea.
3
Gachon Medical Research Institute, Gachon University Gil Medical Center, Incheon, Korea.
4
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.
5
Department of Ophthalmology, Gil Medical Center, Gachon University College of Medicine, Incheon, Korea.

Abstract

PURPOSE:

To evaluate the therapeutic effects of topical 8-oxo-2'-deoxyguanosine (8-oxo-dG) on experimental ocular chemical injury models.

METHODS:

We created ocular chemical injury models with 8-week-old BALB/c mice (n = 70) by applying 100% ethanol; the mice were then treated with 8-oxo-dG eye drops 10 and 5 mg/mL and phosphate-buffered saline (PBS) twice daily. After 7 days, clinical findings such as corneal integrity, clarity, and neovascularization were assessed. Histology, immunohistochemistry findings, and inflammatory cytokine levels using real-time polymerase chain reactions in the corneas of the mice were also analyzed.

RESULTS:

Topical application of 8-oxo-dG eye drops resulted in a significant improvement of epithelial defects and clarity, dose dependently (each P < 0.001). Inflammatory cell infiltration and corneal stromal edema were also decreased in the 8-oxo-dG-treated mice compared with PBS-treated controls, based on hematoxylin and eosin staining. The expressions of F4/80 and neutrophil elastase-positive inflammatory cells and IL-1 and TNF-α cytokine levels were significantly reduced in the 8-oxo-dG group compared with the PBS group (each P < 0.01).

CONCLUSIONS:

Topical 8-oxo-dG application showed an excellent therapeutic effect in ocular chemical injury models by suppressing inflammation.

PMID:
29923862
DOI:
10.1097/ICO.0000000000001671
[Indexed for MEDLINE]

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