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Bull Cancer. 2018 Jul - Aug;105(7-8):643-651. doi: 10.1016/j.bulcan.2018.05.003. Epub 2018 Jun 18.

Exosomes of glioma cells deliver miR-148a to promote proliferation and metastasis of glioblastoma via targeting CADM1.

Author information

1
Department of Pediatrics, the Third Xiangya Hospital, Central South University, 410013 Changsha, China.
2
Department of Neurology, the Third Xiangya Hospital, Central South University, 410013 Changsha, China. Electronic address: zhuandingxym@163.com.
3
Department of Anesthesiology, the Third Xiangya Hospital, Central South University, 410013 Changsha, China.

Abstract

Exosomes are now considered to be involved in mediating cell-to-cell communication to promote or inhibit tumor progression. However, the role and molecular mechanism of exosomes in promoting glioblastoma (GBM) metastasis remains elusive. Here, we found that circulating exosomal miR-148a levels were significantly higher in serum from GBM patients compared with serum from healthy volunteers. In T98G cells, inhibition of miR-148a suppressed cell proliferation and metastasis. In addition, we identified Cell adhesion molecule 1 (CADM1) as a target gene of miR-148a using luciferase reporter assay. Both protein and mRNA levels of CADM1 were decreased in tissues from GBM patients. There was a strong negative correlation between exosomal miR-148a and CADM1 mRNA levels in samples of patients. Moreover, miR-148a antagonist increased p-STAT3 protein level to activate STAT3 pathway. In conclusion, our findings indicated that miR-148a delivered by exosomes may promote cancer cell proliferation and metastasis via targeting CADM1 to activate STAT3 pathway, suggesting a predictor and therapeutic target role of exosomal miR-148a in GBM patients.

KEYWORDS:

CADM1; Cellules de gliome; Glioma cells; Metastasis; Métastase; Proliferation; Prolifération; miR-148a

PMID:
29921422
DOI:
10.1016/j.bulcan.2018.05.003
[Indexed for MEDLINE]
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