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J Biol Regul Homeost Agents. 2018 May-Jun;32(3):506-516.

miR-145 is critical for modulation of vascular smooth muscle cell proliferation in human carotid artery stenosis.

Han Z1,2, Hu H3, Yin M4,5, Li X4, Li J6, Liu L1,2, Liu B1.

Author information

1
Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
2
Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
3
Department of Vascular and Thyroid Surgery, the First Hospital of China Medical University, Shenyang, China.
4
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.
5
Pathology and the Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, USA.
6
Department of Endodontics, the First Affiliated Hospital of Harbin Medical University, Harbin, China.

Abstract

miR-145 is highly expressed in vascular cells, where it regulates phenotypic switching and vascular homeostasis, but its role in carotid artery stenosis (CAS) is controversial. In the present study, the expression of miR-145 was assessed by real time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in human samples (both plasma and/or endarterectomy samples) from patients with symptomatic CAS and in controls without CAS. The mouse carotid artery ligation (CAL) model was used to determine the role of miR-145 on vascular smooth muscle cells in vivo (VSMCs) by using a mimic of or an inhibitor of miR-145. We found that miR-145 expression was significantly reduced in the plasma and plaque from patients with CAS (p less than 0.01). The expression of miR-145 in the mouse CAL model, as assessed by qRT-PCR, was significantly reduced compared to the carotid arteries of the control group (p less than 0.01). In vitro, enhancement or inhibition of miR-145 in VSMCs demonstrated that miR-145 significantly inhibited proliferation of VSMCs (p less than 0.05); in vivo, enhancement of miR-145 significantly inhibited neointimal formation in the CAL model (p less than 0.01). These results demonstrate that the expression of miR-145 is reduced in human CAS, miR-145 plays a critical role in CAS by modulation of VSMC proliferation, suggesting that MiR-145 may present a potential therapeutic option for treating CAS.

PMID:
29921375
[Indexed for MEDLINE]

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