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J Agric Food Chem. 2018 Jul 11;66(27):6997-7005. doi: 10.1021/acs.jafc.8b00047. Epub 2018 Jul 2.

Untargeted 1H NMR-Based Metabolomics Analysis of Urine and Serum Profiles after Consumption of Lentils, Chickpeas, and Beans: An Extended Meal Study To Discover Dietary Biomarkers of Pulses.

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Biomarkers & Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, XaRTA, INSA , Faculty of Pharmacy and Food Sciences, University of Barcelona , Barcelona 08028 , Spain.
CIBER de Fragilidad y Envejecimiento Saludable (CIBERFES) , Instituto de Salud Carlos III , Barcelona , Spain.
Department of Biology and Biological Engineering, Food and Nutrition Science , Chalmers University of Technology , Gothenburg SE-412 96 , Sweden.
Department of Molecular Sciences , Swedish University of Agricultural Sciences , Uppsala 750 07 , Sweden.


High legume intake has been shown to have beneficial effects on the health of humans. The use of nutritional biomarkers, as a complement to self-reported questionnaires, could assist in evaluating dietary intake and downstream effects on human health. The aim of this study was to investigate potential biomarkers of the consumption of pulses (i.e., white beans, chickpeas, and lentils) by using untargeted NMR-based metabolomics. Meals rich in pulses were consumed by a total of 11 participants in a randomized crossover study and multilevel partial least-squares regression was employed for paired comparisons. Metabolomics analysis indicated that trigonelline, 3-methylhistidine, dimethylglycine, trimethylamine, and lysine were potential, though not highly specific, biomarkers of pulse intake. Furthermore, monitoring of these metabolites for a period of 48 h after intake revealed a range of different excretion patterns among pulses. Following the consumption of pulses, a metabolomic profiling revealed that the concentration ratios of trigonelline, choline, lysine, and histidine were similar to those found in urine. In conclusion, this study identified potential urinary biomarkers of exposure to dietary pulses and provided valuable information about the time-response effect of these putative biomarkers.


NMR; biomarkers; dietary pulses; legumes; metabolomics

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