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Artif Cells Nanomed Biotechnol. 2018;46(sup2):1120-1126. doi: 10.1080/21691401.2018.1479711. Epub 2018 Jun 19.

MicroRNA-1297 contributes to the progression of human cervical carcinoma through PTEN.

Author information

1
a The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
2
b Women&infants Hospital Of Zhengzhou , Zhengzhou , China.
3
c National Institutes of Health , Bethesda , MD , USA.

Abstract

BACKGROUND:

The human cervical carcinoma oncogenic mechanisms still remain elusive. Thus, we proposed to understand the biological role of a newly discovered therapeutic miRNA.

METHODS:

MiR-1297 related to human cervical carcinoma was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA, phosphatase and tensin homolog (PTEN) expression in randomly chosen tumour with non-tumour tissues, and the apoptosis factors expression. Cell proliferation was monitored by CCK-8 assay and colony formation assay. Apoptosis was determined by flow cytometry. Protein level was determined by western blotting. 3'UTR was performed to validate the direct binding sites of miR-1297 on PTEN. SPSS was used for statistical analyses.

RESULTS:

MiR-1297 is repressed and PTEN activated in human cervical cancer tissues. After miR-1297 overexpression, HeLa cells had an increase in cell proliferation and decrease in apoptosis. PTEN expression is negatively correlation with miR-1297. PTEN silencing display the similar pattern as miRNA-1297 overexpression to inhibit HeLa cell growth and apoptosis in vitro.

CONCLUSIONS:

Our data indicate that miR-1297 contribute to the human cervical carcinoma through PTEN. miR-1297 could be a reasonable miRNA for future studies.

KEYWORDS:

MiR-1297; PTEN; apoptosis; cell proliferation

PMID:
29916735
DOI:
10.1080/21691401.2018.1479711
[Indexed for MEDLINE]

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