Format

Send to

Choose Destination
Methods Mol Biol. 2018;1774:555-570. doi: 10.1007/978-1-4939-7802-1_25.

Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.

Author information

1
Food Safety & Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute (CFTRI), Mysore, 570020, Karnataka, India. kannan.rf0829@cftri.res.in.
2
Institute of Stem Cell Biology and Regenerative Medicine, National Centre for Biological Sciences, GKVK, Bangalore, India. kannan.rf0829@cftri.res.in.
3
Institute of Stem Cell Biology and Regenerative Medicine, National Centre for Biological Sciences, GKVK, Bangalore, India.

Abstract

Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of regenerating large portions of missing body parts. It is one of the best model systems available to address the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from planarians is imperative to understand their role in the regeneration processes. Here we describe a stable isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters (NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact and regenerating planarians.

KEYWORDS:

Mass spectrometry; Neurotransmitters; Planaria; Quantification; Regeneration

PMID:
29916178
DOI:
10.1007/978-1-4939-7802-1_25
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center