Format

Send to

Choose Destination
Methods Mol Biol. 2018;1774:423-434. doi: 10.1007/978-1-4939-7802-1_15.

Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.

Author information

1
The Scripps Research Institute, La Jolla, CA, USA.
2
Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, MO, USA. asa@stowers.org.

Abstract

Detection of cell proliferation based on the incorporation of 5'-bromo-2'-deoxyuridine (BrdU) has become a standard approach for studying stem cell and progenitor cell populations in developing and adult tissue. In this chapter, we describe three BrdU administration methods for planarians and a staining protocol combining BrdU detection with whole-mount fluorescence in situ hybridization (FISH). Collectively, these protocols enable the combined analysis of BrdU-incorporation and endogenous gene expression, as for example during lineage tracing applications.

KEYWORDS:

BrdU; Cell proliferation; Fluorescence in situ hybridization; Immunostaining; Planarians; Whole-mount

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center