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Methods Mol Biol. 2018;1774:423-434. doi: 10.1007/978-1-4939-7802-1_15.

Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.

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The Scripps Research Institute, La Jolla, CA, USA.
Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, MO, USA.


Detection of cell proliferation based on the incorporation of 5'-bromo-2'-deoxyuridine (BrdU) has become a standard approach for studying stem cell and progenitor cell populations in developing and adult tissue. In this chapter, we describe three BrdU administration methods for planarians and a staining protocol combining BrdU detection with whole-mount fluorescence in situ hybridization (FISH). Collectively, these protocols enable the combined analysis of BrdU-incorporation and endogenous gene expression, as for example during lineage tracing applications.


BrdU; Cell proliferation; Fluorescence in situ hybridization; Immunostaining; Planarians; Whole-mount

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