Format

Send to

Choose Destination
Aging (Albany NY). 2018 Jun 18;10(6):1390-1401. doi: 10.18632/aging.101475.

Five ETS family members, ELF-1, ETV-4, ETV-3L, ETS-1, and ETS-2 upregulate human leukocyte-associated immunoglobulin-like receptor-1 gene basic promoter activity.

Cao Q#1,2, Yang S#3, Lv Q#2,4, Liu Y1, Li L5, Wu X1, Qu G2,4, He X6, Zhang X1, Sun S4, Li B7, An J8, Hu T1, Xue J1.

Author information

1
Department of Immunology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China.
2
Anti-aging Research Institution, Binzhou Medical University, Shandong 264003, China.
3
School of Agriculture, Ludong University, Shandong 264003, China.
4
School of Gerontology, Binzhou Medical University, Shandong 264003, China.
5
Beijing Key Laboratory of Drug Target and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
6
The People's Liberation Army 107 Hospital, Affiliated Hospital of Bin Zhou Medical University, Yantai 264002, China.
7
Department of Microbiology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China.
8
School of Medicine, University of California - San Diego, La Jolla, CA 92037, USA.
#
Contributed equally

Abstract

Human leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1), an immunoinhibitory receptor, is expressed on most types of hematopoietic cells and some tumor cells. LAIR-1 plays an inhibitory role in immune cell maturation, differentiation, and activation. LAIR-1 is also involved in some autoimmune diseases and tumors. However, the mechanism controlling the regulation of the LAIR-1 gene is still unknown. In order to elucidate the molecular mechanisms involved in LAIR-1 regulation, in the present study, we cloned and characterized the promoter region of LAIR-1 gene using a series of truncated promoter plasmids in luciferase reporter assays. Our results show that the basic core promoter of LAIR-1 is located within the region -256/-8 relative to the translational start site. Our further studies indicate that five ETS transcription factors: ELF-1, ETV-4, ETV-3L, ETS-1 and ETS-2, can up-regulate the LAIR-1 basic promoter activity. Of these, ETS-2 is the most effective transcription factor. Moreover, ETS-2 was confirmed to interact directly with the basic promoter of LAIR-1. This study presents the first description of regions/factors capable of up-regulation the promoter activity of LAIR-1. This new knowledge contributes to understanding of the molecular mechanisms involved in LAIR-1 associated immune regulation and diseases.

KEYWORDS:

ETS transcription factors; ETS-2; LAIR-1; Luciferase; promoter; transfection

Supplemental Content

Full text links

Icon for Impact Journals, LLC Icon for PubMed Central
Loading ...
Support Center