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Aging (Albany NY). 2018 Jun 18;10(6):1390-1401. doi: 10.18632/aging.101475.

Five ETS family members, ELF-1, ETV-4, ETV-3L, ETS-1, and ETS-2 upregulate human leukocyte-associated immunoglobulin-like receptor-1 gene basic promoter activity.

Cao Q#1,2, Yang S#3, Lv Q#2,4, Liu Y1, Li L5, Wu X1, Qu G2,4, He X6, Zhang X1, Sun S4, Li B7, An J8, Hu T1, Xue J1.

Author information

Department of Immunology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China.
Anti-aging Research Institution, Binzhou Medical University, Shandong 264003, China.
School of Agriculture, Ludong University, Shandong 264003, China.
School of Gerontology, Binzhou Medical University, Shandong 264003, China.
Beijing Key Laboratory of Drug Target and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
The People's Liberation Army 107 Hospital, Affiliated Hospital of Bin Zhou Medical University, Yantai 264002, China.
Department of Microbiology, School of Basic Medical Sciences, Binzhou Medical University, Shandong 264003, China.
School of Medicine, University of California - San Diego, La Jolla, CA 92037, USA.
Contributed equally


Human leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1), an immunoinhibitory receptor, is expressed on most types of hematopoietic cells and some tumor cells. LAIR-1 plays an inhibitory role in immune cell maturation, differentiation, and activation. LAIR-1 is also involved in some autoimmune diseases and tumors. However, the mechanism controlling the regulation of the LAIR-1 gene is still unknown. In order to elucidate the molecular mechanisms involved in LAIR-1 regulation, in the present study, we cloned and characterized the promoter region of LAIR-1 gene using a series of truncated promoter plasmids in luciferase reporter assays. Our results show that the basic core promoter of LAIR-1 is located within the region -256/-8 relative to the translational start site. Our further studies indicate that five ETS transcription factors: ELF-1, ETV-4, ETV-3L, ETS-1 and ETS-2, can up-regulate the LAIR-1 basic promoter activity. Of these, ETS-2 is the most effective transcription factor. Moreover, ETS-2 was confirmed to interact directly with the basic promoter of LAIR-1. This study presents the first description of regions/factors capable of up-regulation the promoter activity of LAIR-1. This new knowledge contributes to understanding of the molecular mechanisms involved in LAIR-1 associated immune regulation and diseases.


ETS transcription factors; ETS-2; LAIR-1; Luciferase; promoter; transfection

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