Format

Send to

Choose Destination
Biophys J. 2018 Jul 3;115(1):22-25. doi: 10.1016/j.bpj.2018.05.024. Epub 2018 Jun 15.

Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.

Author information

1
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California.
2
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California. Electronic address: sopella@ucsd.edu.

Abstract

Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30 nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.

PMID:
29914645
PMCID:
PMC6035293
[Available on 2019-07-03]
DOI:
10.1016/j.bpj.2018.05.024

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center