Format

Send to

Choose Destination
AMB Express. 2018 Jun 16;8(1):98. doi: 10.1186/s13568-018-0629-9.

The influence of in vitro pectin fermentation on the human fecal microbiome.

Author information

1
Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea.
2
Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea.
3
Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea.
4
Research Group of Food Processing, Korea Food Research Institute, Wanju, 55365, Republic of Korea.
5
Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea. cspark@khu.ac.kr.
6
Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea. sdh83@kfri.re.kr.

Abstract

Pectin is a complex dietary fiber and a prebiotic. To investigate pectin-induced changes in the gut microbiome and their effects on the short chain fatty acids (SCFAs) production, we performed in vitro pectin fermentation using the feces of three Korean donors. The pectin degradations in all three donors were observed. While the donors displayed differences in baseline gut microbiota composition, commonly increased bacteria after pectin fermentation included Lachnospira, Dorea, Clostridium, and Sutterella. Regarding SCFAs, acetate levels rapidly increased with incubation with pectin, and butyrate levels also increased after 6 h of incubation. The results suggest that pectin fermentation increases bacterial species belonging to Clostridium cluster XIV (Lachnospira, Dorea, and Clostridium), with Lachnospira displaying the greatest increase. The results also confirm that pectin fermentation leads to the production of acetate and butyrate.

KEYWORDS:

Fecal microbiota; Fermentation; Pectin; Prebiotic; Short chain fatty acids

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center