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Materials (Basel). 2018 Jun 14;11(6). pii: E1013. doi: 10.3390/ma11061013.

Modified Polymeric Nanoparticles Exert In Vitro Antimicrobial Activity Against Oral Bacteria.

Author information

1
Dental School, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain. toledano@correo.ugr.es.
2
College of Dentistry, University of Tennessee Health Science Center, 875 Union Avenue, Memphis, TN 381632110, USA. jbabu@uthsc.edu.
3
Dental School, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain. rosorio@ugr.es.
4
NanoMyP, Spin-Off Enterprise from University of Granada, Edificio BIC-Granada, Av. Innovación 1, Armilla, 18016 Granada, Spain. amedina@nanomyp.com.
5
College of Dentistry, University of Tennessee Health Science Center, 875 Union Avenue, Memphis, TN 381632110, USA. fgarciagodoy@gmail.com.
6
Dental School, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain. toledano@ugr.es.

Abstract

Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis, Lactobacillus lactis, Streptoccocus mutans, gordonii and sobrinus were grown and the number of bacteria was determined by optical density. Nanoparticles were suspended in phosphate-buffered saline (PBS) at 10, 1 and 0.1 mg/mL and incubated with 1.0 mL of each bacterial suspension for 3, 12, and 24 h. The bacterial viability was assessed by determining their ability to cleave the tetrazolium salt to a formazan dye. Data were analyzed by ANOVA and Scheffe&rsquo;s F (p < 0.05). Doxycycline doping efficacy was 70%. A burst liberation effect was produced during the first 7 days. After 21 days, a sustained release above 6 &micro;g/mL, was observed. Calcium and zinc liberation were about 1 and 0.02 &micro;g/mL respectively. The most effective antibacterial material was found to be the Dox-Nanoparticles (60% to 99% reduction) followed by Ca-Nanoparticles or Zn-Nanoparticles (30% to 70% reduction) and finally the non-doped nanoparticles (7% to 35% reduction). P. gingivalis, S. mutans and L. lactis were the most susceptible bacteria, being S. gordonii and S. sobrinus the most resistant to the tested nanoparticles.

KEYWORDS:

antibacterial; calcium; doxycycline; nanoparticles; zinc

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