Format

Send to

Choose Destination
Int J Mol Sci. 2018 Jun 14;19(6). pii: E1769. doi: 10.3390/ijms19061769.

Synergistic Antitumor Effect of Oligogalacturonides and Cisplatin on Human Lung Cancer A549 Cells.

Author information

1
Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. abc340414@gmail.com.
2
Department of Leisure, Recreation and Tourism Management, Tzu Hui Institute of Technology, Pingtung 92601, Taiwan. justice8j@gmail.com.
3
Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. hugh0530@gmail.com.
4
Department of Food Technology, Bina Nusantara University, Alam Sutera Campus, Alam Sutera, Tangerang 15143, Indonesia. dione_lovincie@binus.edu.
5
Life Science Center, Hsing Wu University, LinKou District, New Taipei City 24452, Taiwan. 089008@mail.hwu.edu.tw.
6
Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. mcwu@mail.npust.edu.tw.

Abstract

Cisplatin (DPP), a clinically potent antineoplastic agent, is limited by its severe adverse effects. The aim of this study was to investigate the effect of oligogalacturonides (OGA) and DDP on human lung cancer A549 cells. The combined use of OGA and DDP had a synergistic effect on the growth inhibition of A549 cells, changed the cell cycle distribution, and enhanced apoptotic response, especially in sequential combination treatment group of DDP 12 h + OGA 12 h. Western blot analyses showed that the combination treatment of OGA and DDP upregulated Bax, p53, and Caspase-3 and downregulated Bcl-2 proteins. More importantly, DDP-induced toxicity was attenuated by OGA and DDP combination treatment in normal HEK293 cells. Our data suggests that the combined use of OGA from natural sources and DDP could be an important new adjuvant therapy for lung cancer as well as offer important insights for reducing kidney toxicity of DDP and delaying the development of DDP resistance.

KEYWORDS:

adjuvant therapy; cisplatin; oligogalacturonides; synergistic effect

PMID:
29903991
PMCID:
PMC6032352
DOI:
10.3390/ijms19061769
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center