Send to

Choose Destination
Wei Sheng Yan Jiu. 2017 Nov;46(6):960-964.

[Apple polyphenol inhibited the proliferation and migration of breast cancer cell MDA-MB-231 mediated by UHRF1].

[Article in Chinese]

Author information

Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, Suzhou 215123, China.



To investigate the effects of apple polyphenol on cell proliferation and migration of breast cancer cell line MDA-MB-231, and explore the potential mechanism.


The breast cancer cells in the logarithmic phase were treated with 0, 50, 100, 200, 400 and 800 μg/m L of apple polyphenol for 24, 48 and 72 h, then Trypan blue staining was employed to detect cell vitality; CCK8 kit was used to determine cell growth and proliferation; cell migration ability was observed by scratch experiment, and the change of proteins expression level was assessed by Western blot.


Apple polyphenol could content-dependently inhibit breast cancer cell's vitality and proliferation between 0 and 800 μg/m L, decrease the proteins level of ubiquitinlike with PHD and ring finger domain 1( UHRF1) and matrix metalloproteinases-2( MMP2), and the protein levels of DNA methyltransferases 3 a( DNMT3 a) and DNMT3 b, themolecular targets of UHRF1, also decreased. Apple polyphenol inhibition of cell 's migration was more significant between 400 and 800 μg/mL.


Apple polyphenol inhibited the vitality, cell growth and proliferation, and migration of breast cancer cells MDA-MB-231 by decreasing the proteins expression of UHRF1 and MMP2, and the downstream targets of UHRF1, DNMT3 a and DNMT3 b, also decreased.


DNMT3a; DNMT3b; apple polyphenol; breast cancer cell line MDA-MB-231; cell proliferation; matrix metalloproteinases-2; migration; ubiquitinlike with PHD and ring finger domain 1

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center