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Wei Sheng Yan Jiu. 2017 Nov;46(6):960-964.

[Apple polyphenol inhibited the proliferation and migration of breast cancer cell MDA-MB-231 mediated by UHRF1].

[Article in Chinese]

Author information

1
Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, Suzhou 215123, China.

Abstract

OBJECTIVE:

To investigate the effects of apple polyphenol on cell proliferation and migration of breast cancer cell line MDA-MB-231, and explore the potential mechanism.

METHODS:

The breast cancer cells in the logarithmic phase were treated with 0, 50, 100, 200, 400 and 800 μg/m L of apple polyphenol for 24, 48 and 72 h, then Trypan blue staining was employed to detect cell vitality; CCK8 kit was used to determine cell growth and proliferation; cell migration ability was observed by scratch experiment, and the change of proteins expression level was assessed by Western blot.

RESULTS:

Apple polyphenol could content-dependently inhibit breast cancer cell's vitality and proliferation between 0 and 800 μg/m L, decrease the proteins level of ubiquitinlike with PHD and ring finger domain 1( UHRF1) and matrix metalloproteinases-2( MMP2), and the protein levels of DNA methyltransferases 3 a( DNMT3 a) and DNMT3 b, themolecular targets of UHRF1, also decreased. Apple polyphenol inhibition of cell 's migration was more significant between 400 and 800 μg/mL.

CONCLUSION:

Apple polyphenol inhibited the vitality, cell growth and proliferation, and migration of breast cancer cells MDA-MB-231 by decreasing the proteins expression of UHRF1 and MMP2, and the downstream targets of UHRF1, DNMT3 a and DNMT3 b, also decreased.

KEYWORDS:

DNMT3a; DNMT3b; apple polyphenol; breast cancer cell line MDA-MB-231; cell proliferation; matrix metalloproteinases-2; migration; ubiquitinlike with PHD and ring finger domain 1

PMID:
29903207
[Indexed for MEDLINE]

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