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Microbiome. 2018 Jun 14;6(1):107. doi: 10.1186/s40168-018-0492-6.

Saturated long-chain fatty acid-producing bacteria contribute to enhanced colonic motility in rats.

Author information

1
Chinese Medicine Clinical Study Center, Jockey Club School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
2
BGI Genomics, BGI-Shenzhen, Shenzhen, China.
3
Preparatory Office of Shenzhen-Melbourne Institute of Life Sciences and Bioengineering, Guangzhou University of Chinese Medicine, Guangzhou, China.
4
YMU-HKBU Joint Laboratory of Traditional Natural Medicine, Yunnan Minzu University, Kunming, China.
5
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
6
BGI-Shenzhen, Shenzhen, China.
7
Digestive Diseases and Nutrition Center, Department of Pediatrics, The State University of New York at Buffalo, 3435 Main Street, 422BRB, Buffalo, NY, 14214, USA. lixinzhu@buffalo.edu.
8
Chinese Medicine Clinical Study Center, Jockey Club School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. bianzxiang@gmail.com.

Abstract

BACKGROUND:

The gut microbiota is closely associated with gastrointestinal (GI) motility disorder, but the mechanism(s) by which bacteria interact with and affect host GI motility remains unclear. In this study, through using metabolomic and metagenomic analyses, an animal model of neonatal maternal separation (NMS) characterized by accelerated colonic motility and gut dysbiosis was used to investigate the mechanism underlying microbiota-driven motility dysfunction.

RESULTS:

An excess of intracolonic saturated long-chain fatty acids (SLCFAs) was associated with enhanced bowel motility in NMS rats. Heptadecanoic acid (C17:0) and stearic acid (C18:0), as the most abundant odd- and even-numbered carbon SLCFAs in the colon lumen, can promote rat colonic muscle contraction and increase stool frequency. Increase of SLCFAs was positively correlated with elevated abundances of Prevotella, Lactobacillus, and Alistipes. Functional annotation found that the level of bacterial LCFA biosynthesis was highly enriched in NMS group. Essential synthetic genes Fabs were largely identified from the genera Prevotella, Lactobacillus, and Alistipes. Pseudo germ-free (GF) rats receiving fecal microbiota from NMS donors exhibited increased defecation frequency and upregulated bacterial production of intracolonic SLCFAs. Modulation of gut dysbiosis by neomycin effectively attenuated GI motility and reduced bacterial SLCFA generation in the colon lumen of NMS rats.

CONCLUSIONS:

These findings reveal a previously unknown relationship between gut bacteria, intracolonic SLCFAs, and host GI motility, suggesting the importance of SLCFA-producing bacteria in GI motility disorders. Further exploration of this relationship could lead to a precise medication targeting the gut microbiota for treating GI motility disorders.

KEYWORDS:

Gastrointestinal motility disorder; Gut microbiota; Neonatal maternal separation; Saturated long-chain fatty acids

PMID:
29903041
PMCID:
PMC6003035
DOI:
10.1186/s40168-018-0492-6
[Indexed for MEDLINE]
Free PMC Article

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