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Rev Med Inst Mex Seguro Soc. 2018 Mar-Apr;56(2):158-162.

MGIT and other methods for diagnosing tuberculosis in a private hospital system with low incidence

[Article in Spanish; Abstract available in Spanish from the publisher]

Author information

1
TecSalud, Hospital Zambrano Hellion, Unidad de Vigilancia Epidemiológica Hospitalaria. Monterrey, Nuevo León, México jacobo.ayala@medicos.tecsalud.mx

Abstract

in English, Spanish

Background:

Tuberculosis (TB) is still a threat to public health; in 2014 caused 1.5 million deaths worldwide; in hospitals where the prevalence of TB is low, it is appropriate to evaluate the effectiveness of tests to diagnose it.

Methods:

We reviewed reports of airway clinical specimens sent for studies of tuberculosis to the microbiology laboratory from Tec Salud System private hospitals (400 beds) in the metropolitan area of ​​Monterrey, NL, from May 2012 to December 2015. In conjunction with Ziehl-Neelsen (ZN) stain, and solid Lowenstein-Jensen medium (LJ), our laboratory resources also include fluorescent LED microscopy (Carl Zeiss Microscopy Products), MGIT 320 (Mycobacteria Growth Indicator Tube liquid culture detection system, Becton Dickinson), and recently incorporated a real-time PCR system the Xpert MTB/RIF test (Cepheid, Sunnyvale, CA).

Results:

In 731 respiratory specimens from 510 patients, M. tuberculosis was isolated from 78 samples belonging to 50 patients on LJ and MGIT LJ 42 (84%) and MGIT 49, (98%) of the samples. From 41 patients identified, 26 (63.4%) were positive to fluorescein, and 25 (60.9%) with Ziehl-Neelsen stain. Real time PCR (Xpert TB) was positive in 13 out of 17 patient-airway samples (76.4%).

Conclusions:

Usefulness of liquid culture (MGIT) and solid culture (LJ) is shown; the first can significantly cut incubation times.

KEYWORDS:

Mycobacterium Tuberculosis; Tuberculosis; Diagnóstico

PMID:
29901975
[Indexed for MEDLINE]

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