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iScience. 2018 May 25;3:63-85. doi: 10.1016/j.isci.2018.04.007.

Apical Cell-Cell Adhesions Reconcile Symmetry and Asymmetry in Zebrafish Neurulation.

Author information

1
Department of Ophthalmology, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA 15213, USA.
2
Department of Cell and Physiology, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA 15213, USA.
3
Department of Ophthalmology, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA 15213, USA; Department of Developmental Biology, University of Pittsburgh, School of Medicine, 3501 Fifth Avenue, Pittsburgh, PA 15213, USA; Department of Microbiology and Molecular Genetics, University of Pittsburgh, School of Medicine, 3501 Fifth Avenue, Pittsburgh, PA 15213, USA. Electronic address: weix@upmc.edu.

Abstract

The symmetric tissue and body plans of animals are paradoxically constructed with asymmetric cells. To understand how the yin-yang duality of symmetry and asymmetry are reconciled, we asked whether apical polarity proteins orchestrate the development of the mirror-symmetric zebrafish neural tube by hierarchically modulating apical cell-cell adhesions. We found that apical polarity proteins localize by a pioneer-intermediate-terminal order. Pioneer proteins establish the mirror symmetry of the neural rod by initiating two distinct types of apical adhesions: the parallel apical adhesions (PAAs) cohere cells of parallel orientation and the novel opposing apical adhesions (OAAs) cohere cells of opposing orientation. Subsequently, the intermediate proteins selectively augment the PAAs when the OAAs dissolve by endocytosis. Finally, terminal proteins are required to inflate the neural tube by generating osmotic pressure. Our findings suggest a general mechanism to construct mirror-symmetric tissues: tissue symmetry can be established by organizing asymmetric cells opposingly via adhesions.

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