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Nutrients. 2018 Jun 13;10(6). pii: E761. doi: 10.3390/nu10060761.

High-Glucose or -Fructose Diet Cause Changes of the Gut Microbiota and Metabolic Disorders in Mice without Body Weight Change.

Author information

1
Research Division of Food Functionality, Korea Food Research Institute, Jeollabuk-do 55365, Korea. Do.Moon-ho@kfri.re.kr.
2
Research Division of Strategic Food Technology, Korea Food Research Institute, Jeollabuk-do 55365, Korea. ejlee@kfri.re.kr.
3
Research Division of Food Functionality, Korea Food Research Institute, Jeollabuk-do 55365, Korea. Oh.Mi-jin@kfri.re.kr.
4
Research Division of Food Functionality, Korea Food Research Institute, Jeollabuk-do 55365, Korea. kimyus@kfri.re.kr.
5
Research Division of Food Functionality, Korea Food Research Institute, Jeollabuk-do 55365, Korea. hypark@kfri.re.kr.

Abstract

High fat diet-induced changes in gut microbiota have been linked to intestinal permeability and metabolic endotoxemia, which is related to metabolic disorders. However, the influence of a high-glucose (HGD) or high-fructose (HFrD) diet on gut microbiota is largely unknown. We performed changes of gut microbiota in HGD- or HFrD-fed C57BL/6J mice by 16S rRNA analysis. Gut microbiota-derived endotoxin-induced metabolic disorders were evaluated by glucose and insulin tolerance test, gut permeability, Western blot and histological analysis. We found that the HGD and HFrD groups had comparatively higher blood glucose and endotoxin levels, fat mass, dyslipidemia, and glucose intolerance without changes in bodyweight. The HGD- and HFrD-fed mice lost gut microbial diversity, characterized by a lower proportion of Bacteroidetes and a markedly increased proportion of Proteobacteria. Moreover, the HGD and HFrD groups had increased gut permeability due to alterations to the tight junction proteins caused by gut inflammation. Hepatic inflammation and lipid accumulation were also markedly increased in the HGD and HFrD groups. High levels of glucose or fructose in the diet regulate the gut microbiota and increase intestinal permeability, which precedes the development of metabolic endotoxemia, inflammation, and lipid accumulation, ultimately leading to hepatic steatosis and normal-weight obesity.

KEYWORDS:

gut microbiota; high fructose diet; high glucose diet; inflammation; lipid metabolism

PMID:
29899272
PMCID:
PMC6024874
DOI:
10.3390/nu10060761
[Indexed for MEDLINE]
Free PMC Article

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