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MBio. 2018 Jun 12;9(3). pii: e01001-18. doi: 10.1128/mBio.01001-18.

A Single-Cell Approach to the Elusive Latent Human Cytomegalovirus Transcriptome.

Author information

1
University of Arizona, Tucson, Arizona, USA fgoodrum@email.arizona.edu.
2
Oregon Health and Sciences University, Portland, Oregon, USA.

Abstract

Herpesvirus latency has been difficult to understand molecularly due to low levels of viral genomes and gene expression. In the case of the betaherpesvirus human cytomegalovirus (HCMV), this is further complicated by the heterogeneity inherent to hematopoietic subpopulations harboring genomes and, as a consequence, the various patterns of infection that simultaneously exist in a host, ranging from latent to lytic. Single-cell RNA sequencing (scRNA-seq) provides tremendous potential in measuring the gene expression profiles of heterogeneous cell populations for a wide range of applications, including in studies of cancer, immunology, and infectious disease. A recent study by Shnayder et al. (mBio 9:e00013-18, 2018, https://doi.org/10.1128/mBio.00013-18) utilized scRNA-seq to define transcriptomal characteristics of HCMV latency. They conclude that latency-associated gene expression is similar to the late lytic viral program but at lower levels of expression. The study highlights the numerous challenges, from the definition of latency to the analysis of scRNA-seq, that exist in defining a latent transcriptome.

KEYWORDS:

MARS-Seq; cytomegalovirus; herpesviruses; scRNA-seq; transcriptome

PMID:
29895640
PMCID:
PMC6016237
DOI:
10.1128/mBio.01001-18
[Indexed for MEDLINE]
Free PMC Article

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