Format

Send to

Choose Destination
Int J Biol Macromol. 2018 Oct 15;118(Pt A):716-721. doi: 10.1016/j.ijbiomac.2018.06.047. Epub 2018 Jun 9.

l-Arginine and allopurinol supplementation attenuates inflammatory mediators in human osteoblasts-osteoarthritis cells.

Author information

1
The Third Department of Knee Injury, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan Province 471000, China.
2
Department of Orthopedics, Zhengzhou Orthopedics Hospital, Zhengzhou, Henan Province 450000, China.
3
The Third Department of Knee Injury, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan Province 471000, China. Electronic address: HarryaWilsonys@yahoo.com.

Abstract

This study investigated the synergistic effects of l-arginine and allopurinol on antioxidant and inflammatory mediators in human osteoblasts-osteoarthritis (HOb-OA) cells. The cells were treated with allopurinol (50-150 mg/kg bwt) and l-arginine (50-150 mg/kg bwt) for 72 h. Cell viability, catalase, superoxide dismutase (SOD), glutathione peroxidase (Gpx), reduced glutathione (GSH), lipid peroxidation, and the inflammatory markers interleukin 6 (IL-6), interleukin 1β (IL-1β), nuclear factor κB (NF-κB) and tumor necrosis factor alpha (TNF-α) were measured. The combined supplementation with allopurinol and l-arginine increased catalase, SOD, GSH, and Gpx, while it decreased lipid peroxidation, IL-6, IL-1β, and TNF-α. While TNF-α, IL-6, IL-1β, and NF-κB mRNA and protein expression were higher in control HOb-OA cells, the combined supplementation with allopurinol and l-arginine substantially reduced their expression in HOb-OA cells by >40%. In summary, combined supplementation with allopurinol and l-arginine might be very effective in osteoarthritis. A search for therapeutic agents that inhibit inflammation could help to prevent and manage osteoarthritis. However, further studies need to determine the biochemical and molecular mechanisms of these agents in osteoarthritis.

KEYWORDS:

Allopurinol; Inflammation; Lipid peroxidation; Osteoarthritis; l-Arginine

PMID:
29894789
DOI:
10.1016/j.ijbiomac.2018.06.047
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center