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Can J Physiol Pharmacol. 2018 Sep;96(9):963-969. doi: 10.1139/cjpp-2017-0784. Epub 2018 Jun 12.

Effects of CeO2 nanoparticles on the HO-1, NQO1, and GCLC expression in the testes of diabetic rats.

Author information

1
a Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2
b Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
3
c Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Abstract

CeO2 nanoparticles (CNPs) as effective ROS scavengers exhibit potent antioxidant activity. In this study the effect of CNPs investigated was on HO-1, NQO1, and GCLC expression in the streptozotocin (STZ)-induced diabetic rats. Twenty-four male Wistar rats were divided into 4 groups: controls did not receive any treatment; diabetic rats received STZ (60 mg/kg daily); CNPs group received CNPs 30 mg/kg daily for 2 weeks; and rats in STZ + CNPs group received CNPs 30 mg/kg daily for 2 weeks following STZ injection. Oxidative stress was evaluated by measurement of total antioxidant capacity (TAC) and total oxidative status (TOS levels). HO-1, NQO1, and GCLC expression was measured using quantitative real-time PCR. Following STZ injection, significant lower levels of TAC and higher levels of TOS were observed. CNPs could alleviate deleterious effects of diabetes through the enhancement of TAC levels and a significant decline in TOS levels. HO-1, NQO1, and GCLC expression in the diabetic rats were lower than controls. HO-1, NQO1, and GCLC was upregulated in the diabetic rats treated with CNPs. There were significant correlations between NQO1 and GCLC, NQO1 and HO-1, and between HO-1 and GCLC expression. Moreover, Nrf2 was associated with NQO1, GCLC, and HO-1 expression. CNPs as Nrf2 upregulator confer protection against oxidative stress in the testes of STZ-induced diabetic rats by upregulating HO-1, GCLC, and NQO1 cytoprotective genes.

KEYWORDS:

CNPs; GCLC; HO-1; NQO1; Nrf2; diabetes mellitus; diabète sucré; nanoparticules de CeO2 (NPC); oxidative stress; stress oxydatif

PMID:
29894645
DOI:
10.1139/cjpp-2017-0784
[Indexed for MEDLINE]

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