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J Proteome Res. 2018 Aug 3;17(8):2704-2714. doi: 10.1021/acs.jproteome.8b00144. Epub 2018 Jun 29.

Characterization of Metabolomic Profile Associated with Metabolic Improvement after Bariatric Surgery in Subjects with Morbid Obesity.

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Biomarkers & Nutrimetabolomic Lab, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA-UB, Campus Torribera, Pharmacy and Food Science Faculty , University of Barcelona , 08028 Barcelona , Spain.
CIBER Fragilidad y Envejecimiento Saludable [CIBERfes] , Instituto de Salud Carlos III [ISCIII] , 28029 Madrid , Spain.
Biomedical Research Institute [IBIMA], Service of Endocrinology and Nutrition , Malaga Hospital Complex [Virgen de la Victoria] , Campus de Teatinos s/n , 29010 Malaga , Spain.
Genetics, Microbiology and Statistics Department, Biology Faculty , University of Barcelona , 08028 Barcelona , Spain.
Scientific and Technological Centres of the University of Barcelona (CCIT-UB) , 08028 Barcelona , Spain.
Statistics and Bioinformatics Unit , Vall d'Hebron Institut de Recerca [VHIR] , 08035 Barcelona , Spain.
CIBER Fisiopatología de la Obesidad y Nutrición [CIBERobn] , Instituto de Salud Carlos III [ISCIII] , 28029 Barcelona , Spain.


The exact impact of bariatric surgery in metabolically "healthy" (MH) or "unhealthy" (MU) phenotypes for the study of the metabolic improvement is still unknown. We applied an untargeted LC-ESI-TripleTOF-MS-driven metabolomics approach in serum samples from 39 patients with morbid obesity (MH and MU) 1, 3, and 6 months after bariatric surgery. Multiple factor analysis, along with correlation and enrichment analyses, was carried out to distinguish those metabolites associated with metabolic improvement. Hydroxypropionic acids, medium-/long-chain hydroxy fatty acids, and bile acid glucuronides were the most discriminative biomarkers of response between MH and MU phenotypes. Hydroxypropionic (hydroxyphenyllactic-related) acids, amino acids, and glycerolipids were the most significant clusters of metabolites altered after bariatric surgery in MU ( p < 0.001). After surgery, MU and MH changed toward a common metabolic state 3 months after surgery. We observed a negative correlation with changes in waist circumference and cholesterol levels with metabolites of lipid metabolism. Glycemic variables were correlated with hexoses, which, in turn, correlated with gluconic acid and amino acid metabolism. Finally, we noted that hydroxyphenyllactic acid was associated with amino acid and lipid metabolism. Microbial metabolism of amino acid and BA glucuronidation pathways may be the key points of metabolic rearrangement after surgery.


bariatric surgery; hydroxyphenyllactic; indole metabolites; mass spectrometry; metabolically healthy; microbiota metabotype; obesity; untargeted metabolomics

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