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J Cell Physiol. 2018 Jan;234(1):464-474. doi: 10.1002/jcp.26591. Epub 2018 Jun 12.

MiR-124-3p suppresses bladder cancer by targeting DNA methyltransferase 3B.

Zo RB1, Long Z1,2,3.

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Department of Urology Surgery, Shigatse People's Hospital, Shigatse, Tibet, P.R. China.
Department of Gastric Cancer and Soft-Tissue Sarcoma Sugery, Fudan University Shanghai Cancer Center, Shanghai, P.R. China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, P.R. China.


This study was aimed to uncover the effects of miR-124-3p on bladder cancer (BC) by regulating DNA methyltransferase 3B. The expressions of miR-124-3p and DNMT3B mRNA in BC tissues and cell lines were detected using RT-PCR. The expression of DNMT3B in cells was determined using Western blot and immunohistochemistry in tissues. In addition, chromogenic in situ hybridization staining was used to measure the expression of miR-124-3p in tissues. BC cells were transfected with miR-124-3p mimics, miR-124-3p inhibitors, DNMT3B siRNAs, and DNMT3B cDNAs + miR-124-3p mimics. Subsequently, cell proliferation, apoptosis, migration, and invasion were measured using CCK-8, the cytometry test, wound healing assay, and Transwell assay, respectively. Finally, the relationship between miR-124-3p and DNMT3B was confirmed using dual luciferase reporter gene assay. MiR-124-3p expression was significantly lower and the level of DNMT3B was significantly higher in BC tissues and cell lines compared with the normal controls. MiR-124-3p was verified to target DNMT3B. The transfection of miR-124-3p mimics and DNMT3B siRNAs down-regulated BC cell proliferation, migration, and invasion, as well as induced cell apoptosis; miR-124-3p inhibitors promoted BC cell proliferation, migration, invasion, and reduced cell apoptosis; and the effects of DNMT3B cDNAs can be compromised by miR-124-3p mimics. Thus, we concluded that miR-124-3p could suppress the proliferation, migration, invasion, and promote apoptosis of BC cells by targeting DNMT3B.


DNA methyltransferase 3B; MiR-124-3p; apoptosis; bladder cancer; urinary bladder neoplasms


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