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J Cell Physiol. 2018 Jan;234(1):464-474. doi: 10.1002/jcp.26591. Epub 2018 Jun 12.

MiR-124-3p suppresses bladder cancer by targeting DNA methyltransferase 3B.

Zo RB1, Long Z1,2,3.

Author information

1
Department of Urology Surgery, Shigatse People's Hospital, Shigatse, Tibet, P.R. China.
2
Department of Gastric Cancer and Soft-Tissue Sarcoma Sugery, Fudan University Shanghai Cancer Center, Shanghai, P.R. China.
3
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, P.R. China.

Abstract

This study was aimed to uncover the effects of miR-124-3p on bladder cancer (BC) by regulating DNA methyltransferase 3B. The expressions of miR-124-3p and DNMT3B mRNA in BC tissues and cell lines were detected using RT-PCR. The expression of DNMT3B in cells was determined using Western blot and immunohistochemistry in tissues. In addition, chromogenic in situ hybridization staining was used to measure the expression of miR-124-3p in tissues. BC cells were transfected with miR-124-3p mimics, miR-124-3p inhibitors, DNMT3B siRNAs, and DNMT3B cDNAs + miR-124-3p mimics. Subsequently, cell proliferation, apoptosis, migration, and invasion were measured using CCK-8, the cytometry test, wound healing assay, and Transwell assay, respectively. Finally, the relationship between miR-124-3p and DNMT3B was confirmed using dual luciferase reporter gene assay. MiR-124-3p expression was significantly lower and the level of DNMT3B was significantly higher in BC tissues and cell lines compared with the normal controls. MiR-124-3p was verified to target DNMT3B. The transfection of miR-124-3p mimics and DNMT3B siRNAs down-regulated BC cell proliferation, migration, and invasion, as well as induced cell apoptosis; miR-124-3p inhibitors promoted BC cell proliferation, migration, invasion, and reduced cell apoptosis; and the effects of DNMT3B cDNAs can be compromised by miR-124-3p mimics. Thus, we concluded that miR-124-3p could suppress the proliferation, migration, invasion, and promote apoptosis of BC cells by targeting DNMT3B.

KEYWORDS:

DNA methyltransferase 3B; MiR-124-3p; apoptosis; bladder cancer; urinary bladder neoplasms

PMID:
29893409
DOI:
10.1002/jcp.26591

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