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Sheng Wu Gong Cheng Xue Bao. 2018 May 25;34(5):752-760. doi: 10.13345/j.cjb.170389.

[Role and mechanism of FOXG1 in invasion and metastasis of colorectal cancer].

[Article in Chinese; Abstract available in Chinese from the publisher]
Wu H1,2, Qian C1,2, Liu C1,2, Xiang J1,2, Ye D1,2, Zhang Z1,2, Zhang X1,2.

Author information

1
Oncology Department, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
2
Biological Therapy Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

Abstract

in English, Chinese

This study was aimed to investigate the effect of Forkhead Box G1 (FOXG1) on the epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells and the underlying mechanism. For this purpose, FOXG1 lentiviral interference (shRNA) plasmid and expression plasmid were constructed. Western blotting was used to analyze the expression of FOXG1 protein in five CRC cells, namely RKO, SW480, SW620, LoVo and DLD-1. The shRNA fragment of FOXG1 (shFOXG1) was designed and synthesized. Recombinant plasmids were obtained with the aid of DNA recombination technique. Double digestion and sequencing were used to identify the recombinant plasmids, and then lentivirus packaging, purification and stable transfection were carried out. Additionally, stable CRC cell lines were screened out. The changes of FOXG1 knockdown and overexpression efficiency, E-cadherin, Vimentin, Fibronectin, Snail, Twist mRNA and protein were investigated respectively by Western blotting and qRT-PCR analysis. Furthermore, the changes of cell morphology after knockdown and cell migration ability were evaluated respectively with optical microscopy, scratch test and Transwell assay. FOXG1 had the highest protein expression in RKO and the lowest in DLD-1 among the five CRC cells. Compared with those of the control group, the cell morphology in FOXG1 knockdown RKO group was changed from spindle into round or polygonal shape, cell polarization was enhanced and tight junction assembly was acclerated while cell migration distance was noticeably decreased. Moreover, the number of cells invaded and migrated through chambers was significantly reduced. Among these key factors of EMT, the expression of E-cadherin was increased while the expressions of Vimentin, Fibronectin, Snail and Twist were decreased. The opposite was the case in the overexpressed FOXG1 group. The overexpression of FOXG1 in CRC promoted the invasion and metastasis of CRC cells and played a crucial role in regulating the EMT. Thus, FOXG1 might be a novel therapeutic target in CRC treatment.

KEYWORDS:

EMT; FOXG1; colorectal cancer; metastasis

PMID:
29893083
DOI:
10.13345/j.cjb.170389
[Indexed for MEDLINE]
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