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Nat Commun. 2018 Jun 11;9(1):2279. doi: 10.1038/s41467-018-04676-w.

Ependymal cilia beating induces an actin network to protect centrioles against shear stress.

Author information

1
Cilia biology and neurogenesis, Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France.
2
Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kgs, Lyngby, Denmark.
3
Biochimie Biophysique et Biologie Structurale, I2BC, CNRS, 91198, Gif-sur-Yvette, France.
4
UPMC University Paris 06 UM 1127, Inserm U1127, CNRS UMR7225, ICM, Sorbonne Université, Paris, France.
5
Cell Imaging Platform, INSERM US24 Structure Fédérative de Recherche Necker, Paris Descartes Sorbonne Paris Cité University, Paris, France.
6
Laboratoire de Physique Théorique de la Matière Condensée, CNRS/UPMC, Paris, France.
7
Laboratoire Jean Perrin, CNRS/UPMC, Paris, France.
8
Cilia biology and neurogenesis, Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France. spassky@biologie.ens.fr.
9
Cilia biology and neurogenesis, Institut de biologie de l'Ecole normale supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Université Paris, 75005, Paris, France. delgehyr@biologie.ens.fr.

Abstract

Multiciliated ependymal cells line all brain cavities. The beating of their motile cilia contributes to the flow of cerebrospinal fluid, which is required for brain homoeostasis and functions. Motile cilia, nucleated from centrioles, persist once formed and withstand the forces produced by the external fluid flow and by their own cilia beating. Here, we show that a dense actin network around the centrioles is induced by cilia beating, as shown by the disorganisation of the actin network upon impairment of cilia motility. Moreover, disruption of the actin network, or specifically of the apical actin network, causes motile cilia and their centrioles to detach from the apical surface of ependymal cell. In conclusion, cilia beating controls the apical actin network around centrioles; the mechanical resistance of this actin network contributes, in turn, to centriole stability.

PMID:
29891944
PMCID:
PMC5996024
DOI:
10.1038/s41467-018-04676-w
[Indexed for MEDLINE]
Free PMC Article

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