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Sci Rep. 2018 Jun 11;8(1):8907. doi: 10.1038/s41598-018-27097-7.

3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study.

Author information

1
Research Center "Physical Materials Science and Composite Materials", National Research Tomsk Polytechnic University, 634050, Tomsk, Russian Federation.
2
Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
3
Laboratory for Applications of Synchrotron Radiation, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
4
Institute for Applied Computer Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
5
Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
6
Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
7
Reutlingen University, Reutlingen, Germany.
8
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany.
9
Fachbereich Chemie, Philipps-Universität Marburg, Marburg, Germany.
10
Research Center "Physical Materials Science and Composite Materials", National Research Tomsk Polytechnic University, 634050, Tomsk, Russian Federation. feja-mari@yandex.ru.
11
Research Center "Physical Materials Science and Composite Materials", National Research Tomsk Polytechnic University, 634050, Tomsk, Russian Federation. rsurmenev@mail.ru.

Abstract

To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 µm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray µCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of PCL will improve tissue ingrowth in vivo due to hydrophilic SiHA microparticles in combination with randomly oriented and well-aligned PCL microfibers, which mimic the structure of extracellular matrix of bone tissue.

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