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Development. 2018 Jul 2;145(13). pii: dev157222. doi: 10.1242/dev.157222.

Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling.

Author information

1
VIB-KU Leuven Center for Brain and Disease Research, Leuven 3000, Belgium m.dobreva@imperial.ac.uk kirstie.lawson@igmm.ed.ac.uk an.zwijsen@kuleuven.be.
2
Department of Human Genetics, KU Leuven, Leuven 3000, Belgium.
3
VIB-KU Leuven Center for Brain and Disease Research, Leuven 3000, Belgium.
4
Department of Cardiovascular Sciences, KU Leuven, Leuven 3000, Belgium.
5
MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK m.dobreva@imperial.ac.uk kirstie.lawson@igmm.ed.ac.uk an.zwijsen@kuleuven.be.
6
Department of Development and Regeneration, KU Leuven, Leuven 3000, Belgium.
7
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden 2333 ZC, The Netherlands.

Abstract

Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm. Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand amniotic ectoderm posteriorly, while descendants of cells remaining anteriorly later expand amniotic ectoderm from its anterior side. Amniogenesis is abnormal in embryos deficient in the bone morphogenetic protein (BMP) signalling effector SMAD5, with delayed closure of the proamniotic canal, and aberrant amnion and folding morphogenesis. Transcriptomics of individual Smad5 mutant amnions isolated before visible malformations and tetraploid chimera analysis revealed two amnion defect sets. We attribute them to impairment of progenitors of the two main cell populations in amniotic ectoderm and to compromised cuboidal-to-squamous transition of anterior amniotic ectoderm. In both cases, SMAD5 is crucial for expanding amniotic ectoderm rapidly into a stretchable squamous sheet to accommodate exocoelom expansion, axial growth and folding morphogenesis.

KEYWORDS:

Amnion; Amnion fate map; BMP-SMAD; Chorion; Clonal analysis; Extra-embryonic ectoderm; Extra-embryonic–embryonic interface

PMID:
29884675
PMCID:
PMC6053664
DOI:
10.1242/dev.157222
[Indexed for MEDLINE]
Free PMC Article

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