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Retrovirology. 2018 Jun 8;15(1):41. doi: 10.1186/s12977-018-0424-3.

Super-resolution fluorescence microscopy studies of human immunodeficiency virus.

Author information

1
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK. jakub.chojnacki@rdm.ox.ac.uk.
2
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK.
3
Institute of Applied Optics, Friedrich-Schiller-University Jena, Max-Wien Platz 4, 07743, Jena, Germany.
4
Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745, Jena, Germany.

Abstract

Super-resolution fluorescence microscopy combines the ability to observe biological processes beyond the diffraction limit of conventional light microscopy with all advantages of the fluorescence readout such as labelling specificity and non-invasive live-cell imaging. Due to their subdiffraction size (< 200 nm) viruses are ideal candidates for super-resolution microscopy studies, and Human Immunodeficiency Virus type 1 (HIV-1) is to date the most studied virus by this technique. This review outlines principles of different super-resolution techniques as well as their advantages and disadvantages for virological studies, especially in the context of live-cell imaging applications. We highlight the findings of super-resolution based HIV-1 studies performed so far, their contributions to the understanding of HIV-1 replication cycle and how the current advances in super-resolution microscopy may open new avenues for future virology research.

KEYWORDS:

Fluorescence; HIV-1; Human immunodeficiency virus; Nanoscopy; Super-resolution microscopy

PMID:
29884197
PMCID:
PMC5994058
DOI:
10.1186/s12977-018-0424-3
[Indexed for MEDLINE]
Free PMC Article

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