Format

Send to

Choose Destination
J Biotechnol. 2018 Aug 20;280:42-48. doi: 10.1016/j.jbiotec.2018.05.017. Epub 2018 Jun 5.

Design, characterisation and application of alginate-based encapsulated pig liver esterase.

Author information

1
Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Interaktion 1, 33619, Bielefeld, Germany; Chair of Organic Chemistry I, Faculty of Chemistry, Bielefeld University, Universitätsstrasse 25, 33615, Bielefeld, Germany.
2
Chair of Organic Chemistry I, Faculty of Chemistry, Bielefeld University, Universitätsstrasse 25, 33615, Bielefeld, Germany.
3
Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Interaktion 1, 33619, Bielefeld, Germany. Electronic address: anant.patel@fh-bielefeld.de.

Abstract

Encapsulation of hydrolases in biopolymer-based hydrogels often suffers from low activities and encapsulation efficiencies along with high leaching and unsatisfactory recycling properties. Exemplified for the encapsulation of pig liver esterase the coating of alginate and chitosan beads have been studied by creating various biopolymer hydrogel beads. Enzyme activity and encapsulation efficiency were notably enhanced by chitosan coating of alginate beads while leaching remained nearly unchanged. This was caused by the enzymatic reaction acidifying the matrix, which increased enzyme retention through enhanced electrostatic enzyme-alginate interaction but decreased activity through enzyme deactivation. A practical and ready-to-use method for visualising pH in beads during reaction by co-encapsulation of a conventional pH indicator was also found. Our method proves that pH control inside the beads can only be realised by buffering. The resulting beads provided a specific activity of 0.267 μmol ∙ min-1 ∙ mg-1, effectiveness factor 0.88, encapsulation efficiency of 88%, 5% leaching and good recycling properties. This work will contribute towards better understanding and application of encapsulated hydrolases for enzymatic syntheses.

KEYWORDS:

Biocatalysis; Biopolymer; Encapsulation; Esterase; Immobilisation

PMID:
29883594
DOI:
10.1016/j.jbiotec.2018.05.017
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center