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Pan Afr Med J. 2017 Nov 23;28:258. doi: 10.11604/pamj.2017.28.258.11197. eCollection 2017.

Bacteriological profile and antimicrobial susceptibility patterns of urine culture isolates from patients in Ndjamena, Chad.

Author information

1
Department of Medical Microbiology and Immunology, School of Health Sciences-Catholic University of Central Africa, Yaoundé, Cameroon.
2
Ndjamena General Hospital of National Reference, Chad.
3
Yaoundé Military Hospital, Cameroon.

Abstract

Introduction:

Bacteriological profile and antimicrobial susceptibility patterns of urine culture isolates were determined among patients in the Ndjamena General Hospital, a National Reference centre.

Methods:

A cross-sectional study was carried out from July to November 2014. Six hundred and sixty patients were enrolled, to whom a cytobacteriological examination of urine was prescribed. Urine was collected and cultured. Bacterial identification and antimicrobial susceptibility patterns were performed using Vitek 2 compact automated system.

Results:

216 isolates were recovered from patients (age range: 10-90 years). E. coli was the pathogen frequently cultured 128 (59.3%) followed by K. pneumonia 28 (13.0%). Bacteriuria was more present in inpatients (70.4%) compared to outpatients (29.6%). High antibiotic-resistance rate (> 60%) of the total isolates was observed with ampicillin, ciprofloxacin and cephalosporins. Imipeneme (94.9%) displayed satisfactory activity against bacteria isolates. ESBLs phenotype was present in 68/105 (64.7%) of betalactamine resistant isolates. AAC(3)-I and AAC(6')-I enzymes were found respectively in 16/36 (44.4%) and 20/36 (55.6%) of aminoglycosides resistant isolates. Resistance of isolates to quinolones was mainly due to an association of target modification (gyrA and parC), porin reduction and/or efflux mechanisms and was present in 107/213 (49%) of quinolones resistant isolates.

Conclusion:

E. coli is the predominant uropathogen isolated in our setting and there are antibiotic-resistant uropathogens among the studied population. Therefore, routine surveillance of bacterial uropathogens to common used antibiotics must be a continuous process so as to provide physicians with up to date information about the local data of antimicrobial resistance.

KEYWORDS:

Bacteriological profile; antimicrobial susceptibility patterns; urine culture isolates

PMID:
29881501
PMCID:
PMC5989234
DOI:
10.11604/pamj.2017.28.258.11197
[Indexed for MEDLINE]
Free PMC Article

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