Format

Send to

Choose Destination
Sci Rep. 2018 Jun 7;8(1):8722. doi: 10.1038/s41598-018-26928-x.

Reelin and aromatase cooperate in ovarian follicle development.

Author information

1
Institute of Neuroanatomy, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany.
2
Department of Anatomy and Molecular Brain Research, Ruhr-University of Bochum, Universitätstrasse 150, 44780, Bochum, Germany.
3
Institute of Anatomy II, University Medical Department of Cologne, Joseph-Stelzmann Str. 9, 50931, Köln, Germany.
4
Institute of Anatomy, University of Oldenburg, Carl-von-Ossietzky Str. 9-11, 26129, Oldenburg, Germany.
5
Institute of Neuroanatomy, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany. rune@uke.de.

Abstract

Reelin plays an important role in cerebral cortex development and synaptogenesis. In the hippocampus, the neurosteroid estrogen affects reelin expression. In this study we tested a potential crosstalk between estradiol and reelin, thus the possibility of a reelin-induced activation of the estradiol synthesizing enzyme aromatase. As a model system, we used ovaries, which express reelin and are a major source of estradiol. We found that in wild-type mice, reelin and aromatase are expressed in granulosa cells of growing follicles. The expression of reelin varies with the estrus cycle and is highest shortly before ovulation, when estradiol serum levels are at their maximum. In ovaries of reelin-deficient reeler mice, aromatase mRNA and protein are significantly reduced, as evidenced by real-time PCR, western blot analysis, and quantitative immunohistochemistry in granulosa cells of preovulatory follicles. In line with reduced estradiol synthesis, ovarian estrus cycle length is prolonged in reeler mice. Most importantly, treating cultured granulosa cells with recombinant reelin results in significant upregulation of aromatase mRNA and protein and increased secretion of estradiol into the supernatant. Our data provide evidence of a local increase of aromatase expression by reelin. Regarding reproduction, this crosstalk may contribute to follicular stability and counteract luteinization in ovaries.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center