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Nat Commun. 2018 Jun 7;9(1):2205. doi: 10.1038/s41467-018-04504-1.

Parasitic insect-derived miRNAs modulate host development.

Wang ZZ1,2, Ye XQ1,3, Shi M1,2, Li F1,2, Wang ZH1,2, Zhou YN1,2, Gu QJ1,2, Wu XT1,2, Yin CL1,2, Guo DH1,2, Hu RM1,2, Hu NN1,2, Chen T1,2, Zheng BY1,2, Zou JN1,2, Zhan LQ1,2, Wei SJ4, Wang YP1,3, Huang JH1,2, Fang XD5, Strand MR6, Chen XX7,8,9.

Author information

1
Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China.
2
Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, 310058, Hangzhou, China.
3
State Key Lab of Rice Biology, Zhejiang University, 310058, Hangzhou, China.
4
Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, 100097, Beijing, China.
5
BGI-Tech, BGI-Shenzhen, 518083, Shenzhen, China.
6
Department of Entomology, University of Georgia, Athens, GA, 30602, USA.
7
Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China. xxchen@zju.edu.cn.
8
Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, 310058, Hangzhou, China. xxchen@zju.edu.cn.
9
State Key Lab of Rice Biology, Zhejiang University, 310058, Hangzhou, China. xxchen@zju.edu.cn.

Abstract

Parasitic wasps produce several factors including venom, polydnaviruses (PDVs) and specialized wasp cells named teratocytes that benefit the survival of offspring by altering the physiology of hosts. However, the underlying molecular mechanisms for the alterations remain unclear. Here we find that the teratocytes of Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and its associated bracovirus (CvBV) can produce miRNAs and deliver the products into the host via different ways. Certain miRNAs in the parasitized host are mainly produced by teratocytes, while the expression level of miRNAs encoded by CvBV can be 100-fold greater in parasitized hosts than non-parasitized ones. We further show that one teratocyte-produced miRNA (Cve-miR-281-3p) and one CvBV-produced miRNA (Cve-miR-novel22-5p-1) arrest host growth by modulating expression of the host ecdysone receptor (EcR). Altogether, our results show the first evidence of cross-species regulation by miRNAs in animal parasitism and their possible function in the alteration of host physiology during parasitism.

PMID:
29880839
PMCID:
PMC5992160
DOI:
10.1038/s41467-018-04504-1
[Indexed for MEDLINE]
Free PMC Article

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