Format

Send to

Choose Destination
Nucleic Acids Res. 1985 Feb 25;13(4):1239-49.

Characterization of a 3'----5' exonuclease activity in the phage phi 29-encoded DNA polymerase.

Abstract

Purified protein p2 of phage phi 29, characterized as a specific DNA polymerase involved in the initiation and elongation of phi 29 DNA replication, contains a 3'----5' exonuclease active on single-stranded DNA, but not on double-stranded DNA. No 5'----3' exonuclease activity was found. The 3'----5' exonuclease activity was shown to be associated with the DNA polymerase since 1) the two activities were heat-inactivated with identical kinetics and 2) both activities, present in purified protein p2, cosedimented in a glycerol gradient.

PMID:
2987819
PMCID:
PMC341069
DOI:
10.1093/nar/13.4.1239
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center