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Nucleic Acids Res. 1985 Jan 25;13(2):389-99.

The nucleotide sequence recognised by the Escherichia coli D type I restriction and modification enzyme.

Abstract

A type I restriction endonuclease from a new isolate of Escherichia coli (E. coli E166) has been purified and characterised. The enzyme, EcoD, has a recognition sequence similar in overall structure to the previously determined type I enzyme sequences, an exception being that it is degenerate. The sequence is 5'-T-T-A-N-N-N-N-N-N-N-G-T-C-Y-3' 3'-A-A-T-N-N-N-N-N-N-N-C-A-G-R-5' where Y is a pyrimidine, R is a purine and N can be any nucleotide. The enzyme methylates adenosyl residues in both strands of the DNA that are separated by ten base pairs, suggesting that the enzyme interacts with DNA along one face of the helix making contacts in two successive major grooves.

PMID:
2987794
PMCID:
PMC341003
DOI:
10.1093/nar/13.2.389
[Indexed for MEDLINE]
Free PMC Article

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