Mass Spectrometry-Based Metabolomics

Methods Mol Biol. 2018:1775:107-118. doi: 10.1007/978-1-4939-7804-5_10.

Abstract

Metabolomics based on mass spectrometry can provide quantitative and qualitative information of the pool of metabolites (metabolome) present intracellularly or extracellularly in a given biological system. A typical metabolomics workflow requires several key steps such as quick and robust sample preparations with quenching of metabolism, chemical derivatization if needed, instrumental measurement, data-processing with/without database information and further statistical analysis and interpretation. Here, we introduce general metabolomics workflows for global and targeted analyses using gas chromatography or liquid chromatography coupled with mass spectrometers.

Keywords: Data processing; Derivatization; Gas chromatography; Library construction; Liquid chromatography; Mass spectrometry; Metabolite extraction.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, Liquid / methods
  • Gas Chromatography-Mass Spectrometry / methods*
  • Mass Spectrometry / methods*
  • Metabolome / genetics*
  • Metabolomics / methods*