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Nat Commun. 2018 Jun 6;9(1):2132. doi: 10.1038/s41467-018-04437-9.

Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme.

Author information

1
Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization, 2425 Kamimurata, Hitachiohmiya, Ibaraki, 319-2293, Japan. nonishi@affrc.go.jp.
2
Division of Basic Research, Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan. nonishi@affrc.go.jp.
3
Structural Biology Team, Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8602, Japan.
4
Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
5
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan.
6
Radiation Breeding Division, Institute of Crop Science, National Agriculture and Food Research Organization, 2425 Kamimurata, Hitachiohmiya, Ibaraki, 319-2293, Japan.
7
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya, 464-8602, Japan.
8
Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA.
9
Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama, 710-0046, Japan.

Abstract

Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.

PMID:
29875377
PMCID:
PMC5989226
DOI:
10.1038/s41467-018-04437-9
[Indexed for MEDLINE]
Free PMC Article

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