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J Cell Biol. 2018 Jul 2;217(7):2445-2462. doi: 10.1083/jcb.201709009. Epub 2018 Jun 6.

The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion.

Author information

1
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada.
2
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
3
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
4
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
5
Stowers Institute for Medical Research, Kansas City, MO.
6
Department of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
7
Department of Molecular and Integrative Physiology, University of Kansas Medical Centre, Kansas City, KS.
8
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada grant.brown@utoronto.ca.

Abstract

Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae "remodels the structure of chromatin" (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance.

PMID:
29875260
PMCID:
PMC6028538
[Available on 2019-01-02]
DOI:
10.1083/jcb.201709009

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