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Curr Genet. 2018 Jun 5. doi: 10.1007/s00294-018-0850-8. [Epub ahead of print]

Scarless genome editing: progress towards understanding genotype-phenotype relationships.

Elison GL1,2, Acar M3,4,5,6.

Author information

1
Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT, 06511, USA.
2
Systems Biology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA.
3
Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT, 06511, USA. murat.acar@yale.edu.
4
Systems Biology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA. murat.acar@yale.edu.
5
Interdepartmental Program in Computational Biology and Bioinformatics, Yale University, 300 George Street, Suite 501, New Haven, CT, 06511, USA. murat.acar@yale.edu.
6
Department of Physics, Yale University, Prospect Street, New Haven, CT, 06511, USA. murat.acar@yale.edu.

Abstract

The ability to predict phenotype from genotype has been an elusive goal for the biological sciences for several decades. Progress decoding genotype-phenotype relationships has been hampered by the challenge of introducing precise genetic changes to specific genomic locations. Here we provide a comparative review of the major techniques that have been historically used to make genetic changes in cells as well as the development of the CRISPR technology which enabled the ability to make marker-free disruptions in endogenous genomic locations. We also discuss how the achievement of truly scarless genome editing has required further adjustments of the original CRISPR method. We conclude by examining recently developed genome editing methods which are not reliant on the induction of a DNA double strand break and discuss the future of both genome engineering and the study of genotype-phenotype relationships.

KEYWORDS:

CRISPR; Genome editing; Genotype-Phenotype relationships

PMID:
29872908
PMCID:
PMC6281794
[Available on 2019-12-05]
DOI:
10.1007/s00294-018-0850-8

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