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Electrophoresis. 2018 Sep;39(17):2218-2227. doi: 10.1002/elps.201800045. Epub 2018 Jul 3.

Carbon molecular sieve based micro-matrix-solid-phase dispersion for the extraction of polyphenols in pomegranate peel by UHPLC-Q-TOF/MS.

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College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, Zhejiang, P. R. China.
Alibaba Business School, Hangzhou Normal University, Hangzhou, Zhejiang, P. R. China.
Lianyungang Hospital of Traditional Chinese Medicine, Lianyungang, Jiangsu, P. R. China.
Drug Clinical Trial Institution, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P. R. China.


A rapid, simple, and efficient sample extraction method based on micro-matrix-solid-phase dispersion (micro-MSPD) was applied to the extraction of polyphenols from pomegranate peel. Five target analytes were determined by ultra-HPLC coupled with Q-TOF/MS. Carbon molecular sieve (CMS) was firstly used as dispersant to improve extraction efficiency in micro-MSPD. The major micro-MSPD parameters, such as type of dispersant, amount of dispersant, grinding time, and the type and the volume of elution solvents, were studied and optimized. Under optimized conditions, 26 mg of pomegranate peel was dispersed with 32.5 mg of CMS, the grinding time was selected as 90 s, the dispersed sample was eluted with 100 μL of methanol. Results showed that the proposed method was of good linearity for concentrations of analytes against their peak areas (coefficient of determination r2 > 0.990), the LOD was as low as 3.2 ng/mL, and the spiking recoveries were between 88.1 and 106%. Satisfactory results were obtained for the extraction of gallic acid, punicalagin A, punicalagin B, catechin, and ellagic acid from pomegranate peel sample, which demonstrated nice reliability and high sensitivity of this approach.


Carbon molecular sieve; Micro-matrix-solid-phase dispersion; Polyphenols; Pomegranate peel; UHPLC-Q-TOF/MS

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