Format

Send to

Choose Destination
Nat Plants. 2018 Jul;4(7):427-431. doi: 10.1038/s41477-018-0178-x. Epub 2018 Jun 4.

Precision genome engineering through adenine base editing in plants.

Author information

1
Center for Genome Engineering, Institute for Basic Science, Daejeon, Republic of Korea.
2
IBS School, University of Science and Technology, Daejeon, Republic of Korea.
3
Center for Genome Engineering, Institute for Basic Science, Daejeon, Republic of Korea. jskim01@snu.ac.kr.
4
IBS School, University of Science and Technology, Daejeon, Republic of Korea. jskim01@snu.ac.kr.
5
Department of Chemistry, Seoul National University, Seoul, Republic of Korea. jskim01@snu.ac.kr.

Abstract

The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.

PMID:
29867128
DOI:
10.1038/s41477-018-0178-x

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center