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PLoS Negl Trop Dis. 2018 Jun 4;12(6):e0006492. doi: 10.1371/journal.pntd.0006492. eCollection 2018 Jun.

Molecular characterization of Vibrio cholerae responsible for cholera epidemics in Uganda by PCR, MLVA and WGS.

Author information

1
Ministry of Health Uganda, Department of Community Health, Kampala, Uganda.
2
Johns Hopkins Bloomberg School of Public Health, Department of International Health, DOVE Project, Baltimore, Maryland United States of America.
3
University of Maryland School of Medicine, Department of Epidemiology and Public Health, Baltimore, Maryland, United States of America.
4
Uganda National Health Laboratory Services (UNHS/CPHL), Kampala, Uganda.
5
Makerere University, Department of Geography, Kampala, Uganda.
6
Makerere University School of Public Health, College of Health Sciences, Kampala, Uganda.

Abstract

BACKGROUND:

For almost 50 years sub-Saharan Africa, including Uganda, has experienced several outbreaks due to Vibrio cholerae. Our aim was to determine the genetic relatedness and spread of strains responsible for cholera outbreaks in Uganda.

METHODOLOGY/PRINCIPAL FINDINGS:

Sixty-three V. cholerae isolates collected from outbreaks in Uganda between 2014 and 2016 were tested using multiplex polymerase chain reaction (PCR), multi-locus variable number of tandem repeat analysis (MLVA) and whole genome sequencing (WGS). Three closely related MLVA clonal complexes (CC) were identified: CC1, 32% (20/63); CC2, 40% (25/63) and CC3, 28% (18/63). Each CC contained isolates from a different WGS clade. These clades were contained in the third wave of the 7th cholera pandemic strain, two clades were contained in the transmission event (T)10 lineage and other in T13. Analysing the dates and genetic relatedness revealed that V. cholerae genetic lineages spread between districts within Uganda and across national borders.

CONCLUSION:

The V. cholerae strains showed local and regional transmission within Uganda and the East African region. To prevent, control and eliminate cholera, these countries should implement strong cross-border collaboration and regional coordination of preventive activities.

PMID:
29864113
PMCID:
PMC6002109
DOI:
10.1371/journal.pntd.0006492
[Indexed for MEDLINE]
Free PMC Article

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