Format

Send to

Choose Destination
J Proteome Res. 2018 Aug 3;17(8):2581-2589. doi: 10.1021/acs.jproteome.7b00836. Epub 2018 Jun 28.

CharmeRT: Boosting Peptide Identifications by Chimeric Spectra Identification and Retention Time Prediction.

Author information

1
Bioinformatics Research Group , University of Applied Sciences Upper Austria , Softwarepark 11 , 4232 Hagenberg , Austria.

Abstract

Coeluting peptides are still a major challenge for the identification and validation of MS/MS spectra, but carry great potential. To tackle these problems, we have developed the here presented CharmeRT workflow, combining a chimeric spectra identification strategy implemented as part of the MS Amanda algorithm with the validation system Elutator, which incorporates a highly accurate retention time prediction algorithm. For high-resolution data sets this workflow identifies 38-64% chimeric spectra, which results in up to 63% more unique peptides compared to a conventional single search strategy.

KEYWORDS:

MS/MS; chimeric spectra; database search; mixed spectra; retention time prediction; tandem mass spectrometry; validation

PMID:
29863353
PMCID:
PMC6079931
DOI:
10.1021/acs.jproteome.7b00836
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center