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Eur J Clin Microbiol Infect Dis. 2018 Aug;37(8):1585-1587. doi: 10.1007/s10096-018-3288-5. Epub 2018 Jun 3.

A new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter®.

Author information

1
French National Reference Centre for Campylobacters and Helicobacters, Bacteriology Laboratory, CHU Bordeaux, Bordeaux, France.
2
INSERM U1053 BaRITOn, University of Bordeaux, Bordeaux, France.
3
Emergency Department, CHU Bordeaux, Bordeaux, France.
4
Pediatric Emergency Department, CHU Bordeaux, Bordeaux, France.
5
French National Reference Centre for Campylobacters and Helicobacters, Bacteriology Laboratory, CHU Bordeaux, Bordeaux, France. francis.megraud@chu-bordeaux.fr.
6
INSERM U1053 BaRITOn, University of Bordeaux, Bordeaux, France. francis.megraud@chu-bordeaux.fr.

Abstract

Campylobacter enteritis is the most frequent bacterial enteritis including in children. Its diagnosis suffers from the lack of sensitivity and delayed result of culture. Our aim was to test a new PCR-derived method for Campylobacter diagnosis in comparison to a composite reference. Patients presenting to the emergency ward of our hospital with enteric symptoms during the 2016 summer season were included. In addition to culture, an ELISA and an in-house real-time PCR were performed, as well as the new method (Orion GenRead Campylobacter) on all stool specimens. The composite reference used to consider a case positive for Campylobacter was either culture positive and in case of negative culture both the ELISA and real-time PCR positive. One hundred fifty patients were included, 64 being infants or children. There were 29 cases positive by the composite reference, with 19 of the 64 children (29.7%) and 10 of the 86 adults (11.6%). If performed alone, culture would have missed six cases. The Orion GenRead Campylobacter detected all the positives by the composite reference but also 12 cases negative by the composite reference (sensitivity 100%, specificity 90.1%). Given the characteristics of the new method, it can be used as a screening method for Campylobacter detection.

KEYWORDS:

Culture; Diagnosis; ELISA; Enteritis; PCR

PMID:
29862421
DOI:
10.1007/s10096-018-3288-5
[Indexed for MEDLINE]

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