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Materials (Basel). 2018 May 27;11(6). pii: E899. doi: 10.3390/ma11060899.

Effects of Three Calcium Silicate Cements on Inflammatory Response and Mineralization-Inducing Potentials in a Dog Pulpotomy Model.

Kang CM1,2, Hwang J3, Song JS4,5, Lee JH6,7, Choi HJ8,9, Shin Y10,11.

Author information

1
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, 03722, Korea. zezu7@yuhs.ac.
2
Department of Pharmacology, College of Medicine, Yonsei University, Seoul 03722, Korea. zezu7@yuhs.ac.
3
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, 03722, Korea. kntdent@naver.com.
4
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, 03722, Korea. songjs@yuhs.ac.
5
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea. songjs@yuhs.ac.
6
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, 03722, Korea. leejh@yuhs.ac.
7
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea. leejh@yuhs.ac.
8
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, 03722, Korea. choihj88@yuhs.ac.
9
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea. choihj88@yuhs.ac.
10
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea. densys@yuhs.ac.
11
Department of Conservative Dentistry, College of Dentistry, Yonsei University, 50-1 Yonseiro, Seodaemun-Gu, Seoul 03722, Korea. densys@yuhs.ac.

Abstract

This beagle pulpotomy study compared the inflammatory response and mineralization-inducing potential of three calcium silicate cements: ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK, USA), OrthoMTA (BioMTA, Seoul, Korea), and Endocem MTA (Maruchi, Wonju, Korea). Exposed pulp tissues were capped with ProRoot MTA, OrthoMTA, or Endocem MTA. After 8 weeks, we extracted the teeth, then performed hematoxylin-eosin and immunohistochemical staining with osteocalcin and dentin sialoprotein. Histological evaluation comprised a scoring system with eight broad categories and analysis of calcific barrier areas. We evaluated 44 teeth capped with ProRoot MTA (n = 15), OrthoMTA (n = 18), or Endocem MTA (n = 11). Most ProRoot MTA specimens formed continuous calcific barriers; these pulps contained inflammation-free palisading patterns in the odontoblastic layer. Areas of the newly formed calcific barrier were greater with ProRoot MTA than with Endocem MTA (p = 0.006). Although dentin sialoprotein was highly expressed in all three groups, the osteocalcin expression was reduced in the OrthoMTA and Endocem MTA groups. ProRoot MTA was superior to OrthoMTA and Endocem MTA in all histological analyses. ProRoot MTA and OrthoMTA resulted in reduced pulpal inflammation and more complete calcific barrier formation, whereas Endocem MTA caused a lower level of calcific barrier continuity with tunnel defects.

KEYWORDS:

calcific barrier; calcium silicate cements; inflammation; mineralization; odontoblastic layer; pulpal response

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