Format

Send to

Choose Destination
Exp Gerontol. 2018 Sep;110:125-132. doi: 10.1016/j.exger.2018.05.018. Epub 2018 May 31.

Influence of aging in the modulation of epigenetic biomarkers of carcinogenesis after exposure to air pollution.

Author information

1
GCS-Groupement des Hôpitaux de l'Institut Catholique de Lille, Gerontology Clinic, Faculté Libre de Médecine, Lille 59000, France; Unité de Chimie Environnementale et Interactions sur le Vivant (EA4492), Université du Littoral Côte d'Opale, Dunkerque 59140, France. Electronic address: b.fougere@gmail.com.
2
Unité de Chimie Environnementale et Interactions sur le Vivant (EA4492), Université du Littoral Côte d'Opale, Dunkerque 59140, France.
3
University of Lille 2, Biology of Aging (EA2693), Lille 59000, France.
4
University of Lille 2, Biology of Aging (EA2693), Lille 59000, France; Gerontology Clinic, Les Bateliers Geriatric Hospital, Lille University Hospital, Lille 59000, France.
5
Unité de Chimie Environnementale et Interactions sur le Vivant (EA4492), Université du Littoral Côte d'Opale, Dunkerque 59140, France; GCS-Groupement des Hôpitaux de l'Institut Catholique de Lille, Department of Anatomy and Pathology, Faculté Libre de Médecine, Lille 59000, France.
6
Normandie Univ, UNICAEN, ABTE EA4651, Centre de Lutte Contre le Cancer François Baclesse, Caen 14000, France.

Abstract

BACKGROUND:

Classified as carcinogenic to humans by the IARC in 2013, fine air particulate matter (PM2.5) can be inhaled and retained into the lung or reach the systemic circulation. This can cause or exacerbate numerous pathologies to which the elderly are often more sensitive.

METHODS:

In order to estimate the influence of age on the development of early cellular epigenetic alterations involved in carcinogenesis, peripheral blood mononuclear cells sampled from 90 patients from three age classes (25-30, 50-55 and 75-80 years old) were ex vivo exposed to urban PM2.5.

RESULTS:

Particles exposure led to variations in telomerase activity and telomeres length in all age groups without any influence of age. Conversely, P16INK4A gene expression increased significantly with age after exposure to PM2.5. Age could enhance MGMT gene expression after exposure to particles, by decreasing the level of promoter methylation in the oldest people.

CONCLUSION:

Hence, our results demonstrated several tendencies in cells modification depending on age, even if all epigenetic assays were carried out after a limited exposure time allowing only one or two cell cycles. Since lung cancer symptoms appear only at an advanced stage, our results underline the needs for further investigation on the studied biomarkers for early diagnosis of carcinogenesis to improve survival.

KEYWORDS:

Aging; Biomarkers; Epigenotoxicity; PBMC; PM(2.5)

PMID:
29860068
DOI:
10.1016/j.exger.2018.05.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center